Technology | Cellular Reprogramming Inc.

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We're Ready for the Future

We make iPSCs using a proprietary, 3rd-generation mRNA reprogramming system. In contrast to Sendai and episomal reprogramming methods, our technology is ready to go all the way to the clinic. When transfected mRNA is used to express reprogramming factors there's no need to wait weeks or months for vector to clear, no need to screen iPSCs for sporadic genomic integration or other traces of vector, and no residual risk associated with the possibility of false negatives during the screening process. Our radically accelerated system improves on the already impressive speed and productivity advantages of mRNA reprogramming. The fastest and most efficient method of generating iPSCs yet devised, our system brings benefits in the here and now and for the future.

Scientific Publications

1. Shao, Z. et al. Dysregulated protocadherin-pathway activity as an intrinsic defect in induced pluripotent stem cell-derived cortical interneurons from subjects with schizophrenia. Nature Neuroscience (2019).

2. Ni, P. et al. iPSC-derived homogeneous populations of developing schizophrenia cortical interneurons have compromised mitochondrial function. Molecular Psychiatry (2019).

3. Warren and Lin. "mRNA-Based Genetic Reprogramming." Molecular Therapy (2018).

4. Schlaeger et al. "A comparison of non-integrating reprogramming methods." Nature Biotechnology (2014).

5. Durruthy-Durruthy et al. "Rapid and efficient conversion of integration-free human induced pluripotent stem cells to GMP-grade culture conditions." PLOS ONE (2014).

6. Steichen et al. "Messenger RNA versus retrovirus-based induced pluripotent stem cell reprogramming strategies: analysis of genomic integrity." Stem Cells Translational Medicine (2014).

7. Boreström et al. "Footprint-free human induced pluripotent stem cells from articular cartilage with redifferentiation capacity: a first step toward a clinical-grade cell source." Stem Cells Translational Medicine (2014).

8. Warren et al. "Feeder-free derivation of human induced pluripotent stem cells with messenger RNA." Scientific Reports (2012).

9. Warren et al. "Highly efficient reprogramming to pluripotency and directed differentiation of human cells with synthetic modified mRNA." Cell Stem Cell (2010).